Bioactive and Hepatotoxicity Evaluation of Selected Herbal Remedies Commonly used in Abakaliki, Ebonyi State Nigeria using Albino Wistar Rats

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Published: 2025-04-01
Keywords:
Herbal remedies, Bioactive, Hepatotoxicity, Therapeutic, GC-MS analysis

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Chukwuma Joseph Chukwu
Department of Chemistry, Faculty of physical sciences, Alex Ekwueme Federal University, Ndufu Alike, Nigeria
Obinna Oje
Department of Biochemistry, Faculty of biological sciences, Alex Ekwueme Federal University, Ndufu Alike, Nigeria
Anthony Bishop Alieze
Department of Chemistry, Faculty of physical sciences, Alex Ekwueme Federal University, Ndufu Alike, Nigeria
Martin Ineke
Department of Chemistry, Faculty of physical sciences, Alex Ekwueme Federal University, Ndufu Alike, Nigeria
Idara Emmanuel Atiaetuk
Department of Chemistry, Faculty of physical sciences, Alex Ekwueme Federal University, Ndufu Alike, Nigeria
Joseph Isiugo
Department of Chemistry, Faculty of physical sciences, Alex Ekwueme Federal University, Ndufu Alike, Nigeria
Nduka Omaka Omaka
Department of Chemistry, Faculty of physical sciences, Alex Ekwueme Federal University, Ndufu Alike, Nigeria
Dorathy Nwokonkwo
Department of Industrial Chemistry, Faculty of physical sciences, Ebonyi state University Abakaliki, Nigeria.
Omojuwa Temitope Japhet
Department of Chemistry, Faculty of physical sciences, Alex Ekwueme Federal University, Ndufu Alike, Nigeria
Chinedum Emelike
Department of Physiology, Faculty of Basic Medical Sciences, Alex Ekwueme Federal University, Ndufu-Alike, Nigeria.
Rachel Otuu
Department of Biochemistry, Faculty of biological sciences, Alex Ekwueme Federal University, Ndufu Alike, Nigeria

Abstract

There is increase in liver disease as evidence from data in admission rate accounting for about 45.5% of cases associated with the consumption of herbal remedies in Nigeria. This study was conducted to evaluate the bioactive components of five herbal remedies and their related effects on selected hepatic biomarkers in albino Wister rats. Herbal samples were analyzed using gas chromatograph coupled to a mass spectrophotometer (GC-MS). The histo-pathological examination and liver function test were done using six groups of five rat each with control The herbal samples analyzed revealed the presence as well as the percentage total ion concentration (%tic) of the following compounds reported elsewhere as pain killers in samples , HC-1, HC-2, HC-3, HC-4 and HC-5: Ethyl iso allocholate (7.606%), 17- pentatriacontene (7.831%), 3- acetoxy -7,8 Epoxylanostan-11-ol (7.881%), 3- acetoxy -7,8 Epoxylanostan-11-ol (3.636%), 5,16,20 pregnatriene-3 beta, 20-diol diacetate (6.838%), Cholan-24- oic acid, 3-hydroxy-7-oxo (14.898%), Hentriacontane-14, 16-dione (4.092%). The results of the ALT showed that rats administered with HC-1 and HC-5 had increased activities, while the AST activity was found to increase in rats administered with HC-1 and HC-4 at p<05 when compared with the negative control. These results revealed that the integrity of the liver of rats administered with HC – 1, HC – 4, and HC – 5 were compromised. These observations might be responsible for the leaks of some of the liver function parameters into the blood. The rats administered with HC-2 shows moderate intra hepatic inflammation while the once administered with HC-3 shows severe intra hepatic hemorrhage

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Vol. 2 No. 1 (2025)

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Chukwu, C. J., Oje, O., Alieze, A. B., Ineke , M., Atiaetuk, I. E., Isiugo, J., Omaka, N. O., Nwokonkwo, D., Japhet, O. T., Emelike , C., & Otuu, R. (2025). Bioactive and Hepatotoxicity Evaluation of Selected Herbal Remedies Commonly used in Abakaliki, Ebonyi State Nigeria using Albino Wistar Rats. International Journal Of Research And Technopreneurial Innovations, 2(1), 25-38. https://ijrti.com.ng/index.php/home/article/view/54

References

Akao, Y., Maruyama, H., Matsumoto, K., Ohguchi, K., Nishizawa, K., Sakamoto, T., Araki, Y., Mishima, S., & Nozawa, Y. (2003). Cell growth inhibitory effect of cinnamic acid derivatives from propolis on human tumor cell lines. Biological and Pharmaceutical Bulletin, 26(7), 1057–1059.

Akinyemi, O., & Dada, E. (2014). In vivo anti-typhoid activities and proximate analysis of ethanolic leaf extracts of Parquetina nigrescens. Journal of Pharmacy and Biological Sciences, 9(5), 115–123.

Aloke, C., Emelike, C. U., Obasi, N. A., Ogbu, P. N., Edeogu, C. O., Uzomba, C. G., Ekakitie, O., Adeolu, A. I., Okoro, C. C., Okey, B. P., Aninjoku, G. G., & Bature, C. U. (2021). HPLC profiling and studies on Copaifera salikounda methanol leaf extract on phenylhydrazine-induced hematotoxicity and oxidative stress in rats. Arabian Journal of Chemistry, 14(12), 103428.

Auerbach, B. J., Reynolds, S. J., Lamorde, M., Merry, C., Kukunda-Byobona, C., Ocama, P., Semeere, A. S., Ndyanabo, A., Boaz, I., & Kiggundu, V. (2012). Traditional herbal medicine use associated with liver fibrosis in rural Rakai, Uganda. PLoS ONE, 7, e41737. https://doi.org/10.1371/journal.pone.0041737

Awodele, O., Daniel, A., Popoola, T. D., & Salami, E. F. (2013). A study on pharmacovigilance of herbal medicines in Lagos West Senatorial District, Nigeria. International Journal of Risk & Safety in Medicine, 25, 205–217.

Badi, N., Fazelipour, S., Naji, T., Babaei, M., & Kalantari-Hesari, A. (2022). Histomorphometric and biochemical study of liver and thyroid hormones following administration of MoO₃ nanoparticles in female rats. Iranian Journal of Veterinary Medicine, 16(2), 188–201.

Chalasani, N., Fontana, R. J., Bonkovsky, H. L., Watkins, P. B., Davern, T., Serrano, J., Yang, H., & Rochon, J. (2008). Causes, clinical features, and outcomes from a prospective study of drug-induced liver injury in the United States. Gastroenterology, 135, 1924–1934.

Chukwu, C. J., Okafor, C. O., Nwabue, F. I., Afiukwa, J. N., & Ibiam, U. A. (2014). Phytochemical study of the constituents of Napoleona imperialis and its flavourant properties. Journal of Phytochemistry, 8(4), 188–198.

Chukwu, C. J., Okafor, C. O., Nwabue, F. I., Afiukwa, J. N., & Uwakwe, A. (2015). GC–MS determination of bioactive components of Napoleona imperialis. Journal of Current Research in Chemistry, 7(1), 9–13.

Emelike, C. U., Anyaehie, U. S. B., Iyare, E. E., Obike, C. A., Eleazu, C. O., & Chukwuma, C. (2020). Acute and sub-acute toxicity studies on Combretum dolichopetalum leaves. Slovenian Veterinary Research, 57(3), 105–114.

Joshi, C. S., Priya, E. S., & Venkataraman, S. (2007). Acute and subacute studies on the polyherbal anti-diabetic formulation Diakyur in experimental animal model. Journal of Health Science, 53(2), 245–249.

Lalitha, N. (2013). Protecting traditional knowledge in Siddha system of medicine. Journal of Intellectual Property Rights, 18(3), 272–282.

Lin, G., Wang, J. Y., Li, N., Li, M., Gao, H., Ji, Y., Zhang, F., Wang, H., Zhou, Y., & Ye, Y. (2011). Hepatic sinusoidal obstruction syndrome associated with consumption of Gynura segetum. Journal of Hepatology, 54, 666–673.

Loizzo, M. R., Tundis, R., Conforti, F., Saab, A. M., Statti, G. A., & Menichini, F. (2007). Composition and α-amylase inhibitory effect of essential oils from Cedrus libani. Fitoterapia, 78, 323–326.

Mythilypriya, R., Shanthi, P., & Sachdanandam, P. (2007). Oral acute and subacute toxicity studies with Kalpaamruthaa on rats. Journal of Health Science, 53(4), 351–358.

Nwokediuko, S. C., Osuala, P. C., Uduma, U. V., Alaneme, A. K., Onwuka, C. C., & Mesigo, C. (2013). Pattern of liver disease admissions in a Nigerian tertiary hospital. Nigerian Journal of Clinical Practice, 16, 339–342. https://doi.org/10.4103/1119-3077.113458

Ogbonnia, S., Mbaka, G., Igbokwe, N., Anyika, E., Alli, P., & Nwakakwa, N. (2010). Antimicrobial evaluation and toxicity studies of Leone Bitters. Agriculture and Biology Journal of North America, 1(3), 366–376.

Ojekale, A. B., Lawal, O., Lasisi, M. O., Bamgbose, C. A., & Dosu, F. A. (2019). Bioactive compounds in herbal bitter drinks and effects on hepatic biomarkers. Journal of Applied Science and Environmental Management, 23(12), 2139–2142.

Oyevvo, E. B. (2013). Sub-chronic administration of Febi Super Bitters triggered inflammatory responses in male Wistar rats. Journal of Medical Science, 13(8), 692–699.

Osuide, G. E. (2002). Regulation of herbal medicines in Nigeria: The role of NAFDAC. In Advances in Phytomedicine (Vol. 1, pp. 249–258).

Pieme, C. A., Penlap, V. N., Nkegoum, B., Taziebou, C. L., Tekwu, E. M., Etoa, F. X., & Ngongang, J. (2006). Evaluation of acute and subacute toxicities of plant extracts. African Journal of Biotechnology, 5(3), 283–289.

Stickel, F., Patsenker, E., & Schuppan, D. (2005). Herbal hepatotoxicity. Journal of Hepatology, 43, 901–910. https://doi.org/10.1016/j.jhep.2005.08.002

Teschke, R., Wolff, A., Frenzel, C., Schulze, J., & Eickhoff, A. (2012). Herbal hepatotoxicity: A tabular compilation of reported cases. Liver International, 32, 1543–1556.

Wasan, K. M., Najafi, S., Wong, J., & Kwong, M. (2001). Assessing plasma lipid levels and toxicity following phytostanol administration. Journal of Pharmacy & Pharmaceutical Sciences, 4(3), 228–234.

WHO Expert Committee on Drug Dependence. (2021). Forty-third report (WHO Technical Report Series No. 1034). World Health Organization.

Zaher, A. R., Koza-Taylor, P. H., Bell, R., Obert, L., Runnels, H. A., Beebe, J. S., Laskin, M. P., & Slikker, W. (2011). Increased serum enzyme levels associated with Kupffer cell reduction. American Journal of Pathology, 179(1), 240–247.

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