Multi-Antibiotic Resistant Bacteria Associated with Bioremediated Crude Oil Polluted Soil
Keywords:
Multi-antibiotic resistance, Bioremediation, Crude oilAbstract
This study was carried out to isolate and characterize multi-antibiotic resistance bacteria associated with bioremediated crude oil polluted soil. Soil samples were obtained from a bioremediated site and pristine soil of different depths in Eleme Local Government Area of Rivers state. Physico-chemical characterization of soil samples was determined. Isolation of total heterotrophic bacterial and hydrocarbon utilizing bacterial was carried out. Antibiotics susceptibility test of the isolated bacterial species was determined using the disc diffusion method. Biodegradability potential of isolates that were susceptible to antibiotics was determined using the 2,6-dichlorophenolindophenol (DCPIP) redox method. Bacterial count ranged from 1.7 x 105 cfu/g – 2.8 x 107 cfu/g. Total petroleum hydrocarbon concentration for pristine and bioremediated soil was 69.24 mg/kg and 238.9mg/kg respectively. Bacterial isolates from both soils include Bacillus spp., Pseudomonas spp., Burkholderia spp., Streptococcus spp., Staphylococcus spp., Streptomyces spp., Cellulomonas spp. and Nitrobacter spp. Isolates from bioremediated soil had multiantibiotic resistance index (MAR) of 0.1 -1 while isolates from the pristine soil had multiantibiotic resistance index (MAR) of 0.1 -0.4 with the bioremediated soil having isolates with the highest MAR index of 1. Bacillus spp. and Pseudomonas spp. isolated from the pristine soil which were susceptible to antibiotics were able to degrade crude oil. All isolates from bioremediated soil were resistant to one or more antibiotics exposed to. This poses a serious public health risk.
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